Targeting the insulin-like growth factor receptor and Src signaling network for the treatment of non-small cell lung cancer.

ABSTRACT:

Background

Therapeutic interventions in the insulin-like growth factor receptor (IGF-1R) pathway were expected to provide clinical benefits; however, IGF-1R tyrosine kinase inhibitors (TKIs) have shown limited antitumor efficacy, and the mechanisms conveying resistance to these agents remain elusive.

Methods

The expression and activation of the IGF-1R and Src were assessed via the analysis of a publicly available dataset, as well as immunohistochemistry, Western blotting, RT-PCR, and in vitro kinase assays. The efficacy of IGF-1R TKIs alone or in combination with Src inhibitors was analyzed using MTT assays, colony formation assays, flow cytometric analysis, and xenograft tumor models.

Results

The co-activation of IGF-1R and Src was observed in multiple human NSCLC ce

SUBMITTER: Min HY 

PROVIDER: S-EPMC4453276 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature