Cdc42 and sec10 Are Required for Normal Retinal Development in Zebrafish.

ABSTRACT:

Purpose

To characterize the function and mechanisms of cdc42 and sec10 in eye development in zebrafish.

Methods

Knockdown of zebrafish cdc42 and sec10 was carried out using antisense morpholino injection. The phenotype of morphants was characterized by histology, immunohistology, and transmission electron microscopy (TEM). To investigate a synergistic genetic interaction between cdc42 and sec10, we titrated suboptimal doses of cdc42 and sec10 morpholinos, and coinjected both morpholinos. To study trafficking, a melanosome transport assay was performed using epinephrine.

Results

Cdc42 and sec10 knockdown in zebrafish resulted in both abnormal eye development and increased retinal cell death. Cdc42 morphants had a relatively normal retinal structure, aside from the abse

SUBMITTER: Choi SY 

PROVIDER: S-EPMC4453986 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature