Defective sarcoplasmic reticulum-mitochondria calcium exchange in aged mouse myocardium.

ABSTRACT: Mitochondrial alterations are critically involved in increased vulnerability to disease during aging. We investigated the contribution of mitochondria-sarcoplasmic reticulum (SR) communication in cardiomyocyte functional alterations during aging. Heart function (echocardiography) and ATP/phosphocreatine (NMR spectroscopy) were preserved in hearts from old mice (>20 months) with respect to young mice (5-6 months). Mitochondrial membrane potential and resting O2 consumption were similar in mitochondria from young and old hearts. However, maximal ADP-stimulated O2 consumption was specifically reduced in interfibrillar mitochondria from aged hearts. Second generation proteomics disclosed an increased mitochondrial protein oxidation in advanced age. Because energy production and oxidative statu

SUBMITTER: Fernandez-Sanz C 

PROVIDER: S-EPMC4454162 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature