CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.

ABSTRACT: Centrosomes nucleate spindle formation, direct spindle pole positioning, and are important for proper chromosome segregation during mitosis in most animal cells. We previously reported that centromere protein 32 (CENP-32) is required for centrosome association with spindle poles during metaphase. In this study, we show that CENP-32 depletion seems to release centrosomes from bipolar spindles whose assembly they had previously initiated. Remarkably, the resulting anastral spindles function normally, aligning the chromosomes to a metaphase plate and entering anaphase without detectable interference from the free centrosomes, which appear to behave as free asters in these cells. The free asters, which contain reduced but significant levels of CDK5RAP2, show weak interactions with spindle micr

SUBMITTER: Ohta S 

PROVIDER: S-EPMC4454171 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature