Evaluation of two highly-multiplexed custom panels for massively parallel semiconductor sequencing on paraffin DNA.

ABSTRACT:

Background

Aim

Massively parallel sequencing (MPS) holds promise for expanding cancer translational research and diagnostics. As yet, it has been applied on paraffin DNA (FFPE) with commercially available highly multiplexed gene panels (100s of DNA targets), while custom panels of low multiplexing are used for re-sequencing. Here, we evaluated the performance of two highly multiplexed custom panels on FFPE DNA.

Methods

Two custom multiplex amplification panels (B, 373 amplicons; T, 286 amplicons) were coupled with semiconductor sequencing on DNA samples from FFPE breast tumors and matched peripheral blood samples (n samples: 316; n libraries: 332). The two panels shared 37% DNA targets (common or shifted amplicons). Panel performance was evaluated in paired sample gr

SUBMITTER: Kotoula V 

PROVIDER: S-EPMC4454570 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature