Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells.

ABSTRACT:

Background

In recent years, the rapidly advancing field of low-temperature atmospheric pressure plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species.

Method

The cytopathic effect of low-temperature plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H2O2) and staurosporine were used as controls throughout.

Results

Low-temperature plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony

SUBMITTER: Hirst AM 

PROVIDER: S-EPMC4454887 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature