Characterization of conserved tandem donor sites and intronic motifs required for alternative splicing in corticosteroid receptor genes.

ABSTRACT: Alternative splicing events from tandem donor sites result in mRNA variants coding for additional amino acids in the DNA binding domain of both the glucocorticoid (GR) and mineralocorticoid (MR) receptors. We now show that expression of both splice variants is extensively conserved in mammalian species, providing strong evidence for their functional significance. An exception to the conservation of the MR tandem splice site (an A at position +5 of the MR+12 donor site in the mouse) was predicted to decrease U1 small nuclear RNA binding. In accord with this prediction, we were unable to detect the MR+12 variant in this species. The one exception to the conservation of the GR tandem splice site, an A at position +3 of the platypus GRgamma donor site that was predicted to enhance binding of U

SUBMITTER: Rivers C 

PROVIDER: S-EPMC4455001 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature