Inhibition of Cell Differentiation in Bacillus subtilis by Pseudomonas protegens.

ABSTRACT:

Unlabelled

Interspecies interactions have been described for numerous bacterial systems, leading to the identification of chemical compounds that impact bacterial physiology and differentiation for processes such as biofilm formation. Here, we identified soil microbes that inhibit biofilm formation and sporulation in the common soil bacterium Bacillus subtilis. We did so by creating a reporter strain that fluoresces when the transcription of a biofilm-specific gene is repressed. Using this reporter in a coculture screen, we identified Pseudomonas putida and Pseudomonas protegens as bacteria that secrete compounds that inhibit biofilm gene expression in B. subtilis. The active compound produced by P. protegens was identified as the antibiotic and antifungal molecule 2,4-diacetylphlo

SUBMITTER: Powers MJ 

PROVIDER: S-EPMC4455266 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature