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RNA-guided genome editing in Drosophila with the purified Cas9 protein.

ABSTRACT: We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.

SUBMITTER: Lee JS 

PROVIDER: S-EPMC4455777 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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RNA-guided genome editing in Drosophila with the purified Cas9 protein.

Lee Jeong-Soo JS   Kwak Su-Jin SJ   Kim Jungeun J   Kim Ae-Kyeong AK   Noh Hae Min HM   Kim Jin-Soo JS   Yu Kweon K  

G3 (Bethesda, Md.) 20140528 7

We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay. ...[more]

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