An allosteric model for control of pore opening by substrate binding in the EutL microcompartment shell protein.

ABSTRACT: The ethanolamine utilization (Eut) microcompartment is a protein-based metabolic organelle that is strongly associated with pathogenesis in bacteria that inhabit the human gut. The exterior shell of this elaborate protein complex is composed from a few thousand copies of BMC-domain shell proteins, which form a semi-permeable diffusion barrier that provides the interior enzymes with substrates and cofactors while simultaneously retaining metabolic intermediates. The ability of this protein shell to regulate passage of substrate and cofactor molecules is critical for microcompartment function, but the details of how this diffusion barrier can allow the passage of large cofactors while still retaining small intermediates remain unclear. Previous work has revealed two conformations of the EutL

SUBMITTER: Thompson MC 

PROVIDER: S-EPMC4456109 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature