E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish.

ABSTRACT:

Background

Diet-induced hepatic steatosis is highly associated with nonalcoholic fatty liver disease, which is related to the development of metabolic syndrome. While advanced stage nonalcoholic hepatic steatosis and steatohepatitis (NASH) result ultimately in fibrosis and cirrhosis, the molecular basis for lipid droplet formation is poorly understood. Common pathways underlie the pathology of mammalian obesity and the zebrafish diet-induced obesity model (DIO-zebrafish) used in this study.

Methods

Our analysis involved a combination of transcriptome (DNA microarray) and proteome (two-dimensional electrophoresis) methods using liver tissue from DIO-zebrafish to find candidate genes involved in hepatic steatosis. We conducted intraperitoneal injection (i.p.) of morpholino ant

SUBMITTER: Shimada Y 

PROVIDER: S-EPMC4456805 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature