Measles Epidemics Among Children in Vietnam: Genomic Characterization of Virus Responsible for Measles Outbreak in Ho Chi Minh City, 2014.

ABSTRACT:

Background

Measles remains poorly controlled in Southeast Asia, including Vietnam.

Objectives

The aim of this study was to characterize genes of virus responsible for a measles outbreak among children in Vietnam in 2014.

Study design

Throat swab samples were collected from 122 pediatric patients with suspected measles. Furthermore, peripheral blood mononuclear cells (PBMCs) from 31 of these cases were also collected. Measles virus (MV) RNA was obtained directly from the clinical specimens, amplified by PCR, and then the N and H genes were sequenced.

Results

MV RNA was detectable in throat swabs from all 122 patients tested. Positive-strand viral RNA, which indicates the intermediate replicative form of MV, was also detected in PBMCs from all 31 cases from whom

SUBMITTER: Pham VH 

PROVIDER: S-EPMC4457408 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature