Knockdown of WAVE3 impairs HGF induced migration and invasion of prostate cancer cells.

ABSTRACT:

Background

The WASP (Wiskott-Aldrich syndrome protein) and WAVE (WASP Verpolin homologous) family of proteins are structurally related and responsible for regulation of actin polymerization through their interaction with actin related proteins 2&3 (ARP 2/3). WAVE-3 has exhibited an association with disease progression and poorer prognosis of certain malignancies. In the current study, we determined the role of WAVE-3 in hepatocyte growth factor induced cellular changes including cell matrix interaction, invasion and cellular motility, and pathways that may be responsible for the changes in prostate cancer cells.

Methods

We used hammer head ribozymes to knock down the expression of WAVE-3 in PC-3 prostate cancer cell line. In vitro cellular functional assays including growth,

SUBMITTER: Moazzam M 

PROVIDER: S-EPMC4458333 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature