HuR mediates the synergistic effects of angiotensin II and IL-1β on vascular COX-2 expression and cell migration.

ABSTRACT:

Background and purpose

Angiotensin II (AngII) and IL-1β are involved in cardiovascular diseases through the induction of inflammatory pathways. HuR is an adenylate- and uridylate-rich element (ARE)-binding protein involved in the mRNA stabilization of many genes. This study investigated the contribution of HuR to the increased expression of COX-2 induced by AngII and IL-1β and its consequences on VSMC migration and remodelling.

Experimental approach

Rat and human VSMCs were stimulated with AngII (0.1 μM) and/or IL-1β (10 ng · mL(-1)). Mice were infused with AngII or subjected to carotid artery ligation. mRNA and protein levels were assayed by quantitative PCR, Western blot, immunohistochemistry and immunofluorescence. Cell migration was measured by wound healing and transwel

SUBMITTER: Aguado A 

PROVIDER: S-EPMC4459021 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature