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Quantitative analysis of the Escherichia coli proteome.

ABSTRACT: Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.

SUBMITTER: Wisniewski JR 

PROVIDER: S-EPMC4459560 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature

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Quantitative analysis of the Escherichia coli proteome.

Wiśniewski Jacek R JR   Rakus Dariusz D   Rakus Dariusz D  

Data in brief 20140822

Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article. ...[more]

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