Optimized tools for multicolor stochastic labeling reveal diverse stereotyped cell arrangements in the fly visual system.

ABSTRACT: We describe the development and application of methods for high-throughput neuroanatomy in Drosophila using light microscopy. These tools enable efficient multicolor stochastic labeling of neurons at both low and high densities. Expression of multiple membrane-targeted and distinct epitope-tagged proteins is controlled both by a transcriptional driver and by stochastic, recombinase-mediated excision of transcription-terminating cassettes. This MultiColor FlpOut (MCFO) approach can be used to reveal cell shapes and relative cell positions and to track the progeny of precursor cells through development. Using two different recombinases, the number of cells labeled and the number of color combinations observed in those cells can be controlled separately. We demonstrate the utility of MCFO in

SUBMITTER: Nern A 

PROVIDER: S-EPMC4460454 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature