Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

ABSTRACT:

Background

Due to the absence of transcription initiation regulation of protein coding genes transcribed by RNA polymerase II, posttranscriptional regulation is responsible for the majority of gene expression changes in trypanosomatids. Therefore, cataloging the abundance of mRNAs (transcriptome) and the level of their translation (translatome) is a key step to understand control of gene expression in these organisms.

Results

Here we assess the extent of regulation of the transcriptome and the translatome in the Chagas disease causing agent, Trypanosoma cruzi, in both the non-infective (epimastigote) and infective (metacyclic trypomastigote) insect's life stages using RNA-seq and ribosome profiling. The observed steady state transcript levels support constitutive transcripti

SUBMITTER: Smircich P 

PROVIDER: S-EPMC4460968 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature