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Expression, purification and crystallization of a family 55 ?-1,3-glucanase from Chaetomium thermophilum.

ABSTRACT: A ?-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, ? = 93.1° and one molecule in the asymmetric unit.

SUBMITTER: Papageorgiou AC 

PROVIDER: S-EPMC4461330 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum.

Papageorgiou Anastassios C AC   Li Duochuan D  

Acta crystallographica. Section F, Structural biology communications 20150520 Pt 6

A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit. ...[more]

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