Error-prone pcr-based mutagenesis strategy for rapidly generating high-yield influenza vaccine candidates.

ABSTRACT: Vaccination is the primary strategy for the prevention and control of influenza outbreaks. However, the manufacture of influenza vaccine requires a high-yield seed strain, and the conventional methods for generating such strains are time consuming. In this study, we developed a novel method to rapidly generate high-yield candidate vaccine strains by integrating error-prone PCR, site-directed mutagenesis strategies, and reverse genetics. We used this method to generate seed strains for the influenza A(H1N1)pdm09 virus and produced six high-yield candidate strains. We used a mouse model to assess the efficacy of two of the six candidate strains as a vaccine seed virus: both strains provided complete protection in mice against lethal challenge, thus validating our method. Results confirmed th

SUBMITTER: Ye J 

PROVIDER: S-EPMC4461506 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature