Human bone marrow stromal cell confluence: effects on cell characteristics and methods of assessment.

ABSTRACT:

Background aims

Ex vivo expansion and serial passage of human bone marrow stromal cells (BMSCs, also known as bone marrow-derived mesenchymal stem cells) is required to obtain sufficient quantities for clinical therapy. The BMSC confluence criteria used to determine passage and harvest timing vary widely, and the impact of confluence on BMSC properties remains controversial. The effects of confluence on BMSC properties were studied and confluence-associated markers were identified.

Methods

BMSC characteristics were analyzed as they grew from 50% to 100% confluence, including viability, population doubling time, apoptosis, colony formation, immunosuppression, surface marker expression, global gene expression and microRNA expression. In addition, culture supernatant protein, g

SUBMITTER: Ren J 

PROVIDER: S-EPMC4461557 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature