Genetic Manipulation of NK Cells for Cancer Immunotherapy: Techniques and Clinical Implications.

ABSTRACT: Given their rapid and efficient capacity to recognize and kill tumor cells, natural killer (NK) cells represent a unique immune cell to genetically reprogram in an effort to improve the outcome of cell-based cancer immunotherapy. However, technical and biological challenges associated with gene delivery into NK cells have significantly tempered this approach. Recent advances in viral transduction and electroporation have now allowed detailed characterization of genetically modified NK cells and provided a better understanding for how these cells can be utilized in the clinic to optimize their capacity to induce tumor regression in vivo. Improving NK cell persistence in vivo via autocrine IL-2 and IL-15 stimulation, enhancing tumor targeting by silencing inhibitory NK cell receptors such as

SUBMITTER: Carlsten M 

PROVIDER: S-EPMC4462109 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature