Genome-Wide Identification of Klebsiella pneumoniae Fitness Genes during Lung Infection.

ABSTRACT:

Unlabelled

Klebsiella pneumoniae is an urgent public health threat because of resistance to carbapenems, antibiotics of last resort against Gram-negative bacterial infections. Despite the fact that K. pneumoniae is a leading cause of pneumonia in hospitalized patients, the bacterial factors required to cause disease are poorly understood. Insertion site sequencing combines transposon mutagenesis with high-throughput sequencing to simultaneously screen thousands of insertion mutants for fitness defects during infection. Using the recently sequenced K. pneumoniae strain KPPR1 in a well-established mouse model of pneumonia, insertion site sequencing was performed on a pool of >25,000 transposon mutants. The relative fitness requirement of each gene was ranked based on the ratio of lun

SUBMITTER: Bachman MA 

PROVIDER: S-EPMC4462621 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature