Detection of human immunodeficiency virus type 1 antiretroviral resistance mutations by high-density DNA probe arrays.

ABSTRACT: Genotypic resistance testing has become an important tool in the clinical management of patients infected with human immunodeficiency virus type 1 (HIV-1). Standard sequencing methodology and hybridization-based technology are the two principal methods used for HIV-1 genotyping. This report describes an evaluation of a new hybridization-based HIV-1 genotypic test of 99 clinical samples from patients infected mostly with HIV-1 subtype B and receiving treatment. This test combines RNA extraction with magnetic silica particles, amplification by nested reverse transcriptase PCR, and detection with high-density probe arrays designed to detect 204 antiretroviral resistance mutations simultaneously in Gag cleavage sites, protease, reverse transcriptase, integrase, and gp41. The nested reverse tra

SUBMITTER: Gonzalez R 

PROVIDER: S-EPMC446276 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature