Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ.

ABSTRACT: The rod-shaped Gram-negative bacterium Escherichia coli multiplies by elongation followed by binary fission. Longitudinal growth of the cell envelope and synthesis of the new poles are organized by two protein complexes called elongasome and divisome, respectively. We have analyzed the spatio-temporal localization patterns of many of these morphogenetic proteins by immunolabeling the wild type strain MC4100 grown to steady state in minimal glucose medium at 28°C. This allowed the direct comparison of morphogenetic protein localization patterns as a function of cell age as imaged by phase contrast and fluorescence wide field microscopy. Under steady state conditions the age distribution of the cells is constant and is directly correlated to cell length. To quantify cell size and protein loc

SUBMITTER: Vischer NO 

PROVIDER: S-EPMC4462998 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature