TALE-mediated epigenetic suppression of CDKN2A increases replication in human fibroblasts.

ABSTRACT: Current strategies to alter disease-associated epigenetic modifications target ubiquitously expressed epigenetic regulators. This approach does not allow specific genes to be controlled in specific cell types; therefore, tools to selectively target epigenetic modifications in the desired cell type and strategies to more efficiently correct aberrant gene expression in disease are needed. Here, we have developed a method for directing DNA methylation to specific gene loci by conjugating catalytic domains of DNA methyltransferases (DNMTs) to engineered transcription activator-like effectors (TALEs). We demonstrated that these TALE-DNMTs direct DNA methylation specifically to the targeted gene locus in human cells. Further, we determined that minimizing direct nucleotide sequence repeats withi

SUBMITTER: Bernstein DL 

PROVIDER: S-EPMC4463192 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature