Single cell confocal Raman spectroscopy of human osteoarthritic chondrocytes: a preliminary study.

ABSTRACT: A great deal of effort has been focused on exploring the underlying molecular mechanism of osteoarthritis (OA) especially at the cellular level. We report a confocal Raman spectroscopic investigation on human osteoarthritic chondrocytes. The objective of this investigation is to identify molecular features and the stage of OA based on the spectral signatures corresponding to bio-molecular changes at the cellular level in chondrocytes. In this study, we isolated chondrocytes from human osteoarthritic cartilage and acquired Raman spectra from single cells. Major spectral differences between the cells obtained from different International Cartilage Repair Society (ICRS) grades of osteoarthritic cartilage were identified. During progression of OA, a decrease in protein content and an increase

SUBMITTER: Kumar R 

PROVIDER: S-EPMC4463591 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature