Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae.

ABSTRACT:

Background

Apocarotenoids, like the C13-norisoprenoids, are natural compounds that contribute to the flavor and/or aroma of flowers and foods. They are produced in aromatic plants-like raspberries and roses-by the enzymatic cleavage of carotenes. Due to their pleasant aroma and flavour, apocarotenoids have high commercial value for the cosmetic and food industry, but currently their production is mainly assured by chemical synthesis. In the present study, a Saccharomyces cerevisiae strain that synthesizes the apocarotenoid β-ionone was constructed by combining integrative vectors and high copy number episomal vectors, in an engineered strain that accumulates FPP.

Results

Integration of an extra copy of the geranylgeranyl diphosphate synthase gene (BTS1), together with the ca

SUBMITTER: Lopez J 

PROVIDER: S-EPMC4464609 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature