Development of New Modular Genetic Tools for Engineering the Halophilic Archaeon Halobacterium salinarum.

ABSTRACT: Our ability to genetically manipulate living organisms is usually constrained by the efficiency of the genetic tools available for the system of interest. In this report, we present the design, construction and characterization of a set of four new modular vectors, the pHsal series, for engineering Halobacterium salinarum, a model halophilic archaeon widely used in systems biology studies. The pHsal shuttle vectors are organized in four modules: (i) the E. coli's specific part, containing a ColE1 origin of replication and an ampicillin resistance marker, (ii) the resistance marker and (iii) the replication origin, which are specific to H. salinarum and (iv) the cargo, which will carry a sequence of interest cloned in a multiple cloning site, flanked by universal M13 primers. Each module wa

SUBMITTER: Silva-Rocha R 

PROVIDER: S-EPMC4465625 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature