Cohesin-dependent globules and heterochromatin shape 3D genome architecture in S. pombe.

ABSTRACT: Eukaryotic genomes are folded into three-dimensional structures, such as self-associating topological domains, the borders of which are enriched in cohesin and CCCTC-binding factor (CTCF) required for long-range interactions. How local chromatin interactions govern higher-order folding of chromatin fibres and the function of cohesin in this process remain poorly understood. Here we perform genome-wide chromatin conformation capture (Hi-C) analysis to explore the high-resolution organization of the Schizosaccharomyces pombe genome, which despite its small size exhibits fundamental features found in other eukaryotes. Our analyses of wild-type and mutant strains reveal key elements of chromosome architecture and genome organization. On chromosome arms, small regions of chromatin locally inter

SUBMITTER: Mizuguchi T 

PROVIDER: S-EPMC4465753 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature