Proteomic analysis and functional characterization of mouse brain mitochondria during aging reveal alterations in energy metabolism.

ABSTRACT: Mitochondria are the main cellular source of reactive oxygen species and are recognized as key players in several age-associated disorders and neurodegeneration. Their dysfunction has also been linked to cellular aging. Additionally, mechanisms leading to the preservation of mitochondrial function promote longevity. In this study we investigated the proteomic and functional alterations in brain mitochondria isolated from mature (5 months old), old (12 months old), and aged (24 months old) mice as determinants of normal "healthy" aging. Here the global changes concomitant with aging in the mitochondrial proteome of mouse brain analyzed by quantitative mass-spectrometry based super-SILAC identified differentially expressed proteins involved in several metabolic pathways including glycolysis,

SUBMITTER: Stauch KL 

PROVIDER: S-EPMC4465935 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature