The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.

ABSTRACT: In bacteria the concurrence of DNA replication and transcription leads to potentially deleterious encounters between the two machineries, which can occur in either the head-on (lagging strand genes) or co-directional (leading strand genes) orientations. These conflicts lead to replication fork stalling and can destabilize the genome. Both eukaryotic and prokaryotic cells possess resolution factors that reduce the severity of these encounters. Though Escherichia coli accessory helicases have been implicated in the mitigation of head-on conflicts, direct evidence of these proteins mitigating co-directional conflicts is lacking. Furthermore, the endogenous chromosomal regions where these helicases act, and the mechanism of recruitment, have not been identified. We show that the essential Baci

SUBMITTER: Merrikh CN 

PROVIDER: S-EPMC4466434 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature