Prmt5 is required for germ cell survival during spermatogenesis in mice.

ABSTRACT: During germ cell development, epigenetic modifications undergo extensive remodeling. Abnormal epigenetic modifications usually result in germ cell loss and reproductive defect. Prmt5 (Protein arginine methyltransferase 5) encodes a protein arginine methyltransferase which has been demonstrated to play important roles in germ cell development during embryonic stages. In the present study, we found that Prmt5 was also abundantly expressed in male germ cells after birth. Inactivation of this gene by crossing with Stra8-Cre transgenic mice resulted in germ cell loss during spermatogenesis. Further study revealed that the germ cell development was grossly normal before P10. However, most of the germ cells in Prmt5(Δ/f); Stra8-Cre mice were blocked at meiotic stage. The expression of meiosis ass

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4466585 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature