Three-dimensional Nanowire Structures for Ultra-Fast Separation of DNA, Protein and RNA Molecules.

ABSTRACT: Separation and analysis of biomolecules represent crucial processes for biological and biomedical engineering development; however, separation resolution and speed for biomolecules analysis still require improvements. To achieve separation and analysis of biomolecules in a short time, the use of highly-ordered nanostructures fabricated by top-down or bottom-up approaches have been proposed. Here, we reported on the use of three-dimensional (3D) nanowire structures embedded in microchannels fabricated by a bottom-up approach for ultrafast separation of small biomolecules, such as DNA, protein, and RNA molecules. The 3D nanowire structures could analyze a mixture of DNA molecules (50-1000 bp) within 50 s, a mixture of protein molecules (20-340 kDa) within 5 s, and a mixture of RNA molecules

SUBMITTER: Rahong S 

PROVIDER: S-EPMC4466590 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature