Plastid primers for angiosperm phylogenetics and phylogeography.

ABSTRACT:

Premise of the study

PCR primers are available for virtually every region of the plastid genome. Selection of which primer pairs to use is second only to selection of the genic region. This is particularly true for research at the species/population interface.

Methods

Primer pairs for 130 regions of the chloroplast genome were evaluated in 12 species distributed across the angiosperms. Likelihood of amplification success was inferred based upon number and location of mismatches to target sequence. Intraspecific sequence variability was evaluated under three different criteria in four species.

Results

Many published primer pairs should work across all taxa sampled, with the exception of failure due to genomic reorganization events. Universal barcoding primers were the

SUBMITTER: Prince LM 

PROVIDER: S-EPMC4467757 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature