Metabarcoding of the kombucha microbial community grown in different microenvironments.

ABSTRACT: Introducing of the DNA metabarcoding analysis of probiotic microbial communities allowed getting insight into their functioning and establishing a better control on safety and efficacy of the probiotic communities. In this work the kombucha poly-microbial probiotic community was analysed to study its flexibility under different growth conditions. Environmental DNA sequencing revealed a complex and flexible composition of the kombucha microbial culture (KMC) constituting more bacterial and fungal organisms in addition to those found by cultural method. The community comprised bacterial and yeast components including cultured and uncultivable microorganisms. Culturing the KMC under different conditions revealed the core part of the community which included acetobacteria of two genera Komagat

SUBMITTER: Reva ON 

PROVIDER: S-EPMC4467805 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature