Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection.

ABSTRACT:

Background

Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4.

Methodology/principal findings

Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3´non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. Th

SUBMITTER: Abd El Wahed A 

PROVIDER: S-EPMC4468249 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature