TALEN-mediated apc mutation in Xenopus tropicalis phenocopies familial adenomatous polyposis.

ABSTRACT: Truncating mutations in the tumor suppressor gene adenomatous polyposis coli (APC) are the initiating step in the vast majority of sporadic colorectal cancers, and they underlie familial adenomatous polyposis (FAP) syndromes. Modeling of APC- driven tumor formation in the mouse has contributed substantially to our mechanistic understanding of the associated disease, but additional models are needed to explore therapeutic opportunities and overcome current limitations of mouse models. We report on a novel and penetrant genetic cancer model in Xenopus tropicalis, an aquatic tetrapod vertebrate with external development, diploid genome and short life cycle. Tadpoles and froglets derived from embryos injected with TAL effector nucleases targeting the apc gene rapidly developed intestinal hyper

SUBMITTER: Van Nieuwenhuysen T 

PROVIDER: S-EPMC4468341 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature