Deciphering poxvirus gene expression by RNA sequencing and ribosome profiling.

ABSTRACT:

Unlabelled

The more than 200 closely spaced annotated open reading frames, extensive transcriptional read-through, and numerous unpredicted RNA start sites have made the analysis of vaccinia virus gene expression challenging. Genome-wide ribosome profiling provided an unprecedented assessment of poxvirus gene expression. By 4 h after infection, approximately 80% of the ribosome-associated mRNA was viral. Ribosome-associated mRNAs were detected for most annotated early genes at 2 h and for most intermediate and late genes at 4 and 8 h. Cluster analysis identified a subset of early mRNAs that continued to be translated at the later times. At 2 h, there was excellent correlation between the abundance of individual mRNAs and the numbers of associated ribosomes, indicating that expressi

SUBMITTER: Yang Z 

PROVIDER: S-EPMC4468498 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature