Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages.

ABSTRACT: Salmonella enterica serovar Typhimurium is a common cause of food-borne gastrointestinal illness, but additionally it causes potentially fatal bacteremia in some immunocompromised patients. In mice, systemic spread and replication of the bacteria depend upon infection of and replication within macrophages, but replication in human macrophages is not widely reported or well studied. In order to assess the ability of Salmonella Typhimurium to replicate in human macrophages, we infected primary monocyte-derived macrophages (MDM) that had been differentiated under conditions known to generate different phenotypes. We found that replication in MDM depends greatly upon the phenotype of the cells, as M1-skewed macrophages did not allow replication, while M2a macrophages and macrophages differenti

SUBMITTER: Lathrop SK 

PROVIDER: S-EPMC4468555 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature