High-resolution characterization of sequence signatures due to non-random cleavage of cell-free DNA.

ABSTRACT:

Background

High-throughput sequencing of cell-free DNA fragments found in human plasma has been used to non-invasively detect fetal aneuploidy, monitor organ transplants and investigate tumor DNA. However, many biological properties of this extracellular genetic material remain unknown. Research that further characterizes circulating DNA could substantially increase its diagnostic value by allowing the application of more sophisticated bioinformatics tools that lead to an improved signal to noise ratio in the sequencing data.

Methods

In this study, we investigate various features of cell-free DNA in plasma using deep-sequencing data from two pregnant women (>70X, >50X) and compare them with matched cellular DNA. We utilize a descriptive approach to examine how the biological

SUBMITTER: Chandrananda D 

PROVIDER: S-EPMC4469119 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature