Clinical significance of DNA methylation mRNA levels of TET family members in colorectal cancer.

ABSTRACT:

Purpose

Ten eleven translocation (TET) enzyme activity is essential for active DNA demethylation in biological processes, and their altered expression has been observed in various malignancies. Therefore, we investigated DNA methylation and mRNA levels of all TETs in colorectal cancer (CRC) patients.

Methods

TET mRNA levels were evaluated using quantitative RT-PCR in primary cancerous and histopathologically unchanged colorectal tissues from patients who underwent radical surgical colon resection (n = 113). DNA methylation levels of the TET CpG island were assessed using bisulfite DNA sequencing and high-resolution melting analysis.

Results

We found reduced transcript levels of TET1, TET2 and TET3 in cancerous tissue compared with their histopathologically unchanged c

SUBMITTER: Rawluszko-Wieczorek AA 

PROVIDER: S-EPMC4469774 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature