Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor.

ABSTRACT: Lentiviral vectors are widely used in the field of gene therapy as an effective method for permanent gene delivery. While current methods of producing small scale vector batches for research purposes depend largely on culture flasks, the emergence and popularity of lentiviral vectors in translational, preclinical and clinical research has demanded their production on a much larger scale, a task that can be difficult to manage with the numbers of producer cell culture flasks required for large volumes of vector. To generate a large scale, partially closed system method for the manufacturing of clinical grade lentiviral vector suitable for the generation of induced pluripotent stem cells (iPSCs), we developed a method employing a hollow fiber bioreactor traditionally used for cell expansion.

SUBMITTER: Sheu J 

PROVIDER: S-EPMC4470365 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature