The ionic bases of the action potential in isolated mouse cardiac Purkinje cell.

ABSTRACT:

Background

Collecting electrophysiological and molecular data from the murine conduction system presents technical challenges. Thus, only little advantage has been taken of numerous genetically engineered murine models to study excitation through the cardiac conduction system of the mouse.

Objective

To develop an approach for isolating murine cardiac Purkinje cells (PCs), to characterize major ionic currents and to use the data to simulate action potentials (APs) recorded from PCs.

Methods

Light microscopy was used to isolate and identify PCs from apical and septal cells. Current and voltage clamp techniques were used to record APs and whole cell currents. We then simulated a PC AP on the basis of our experimental data.

Results

APs recorded from PCs were signif

SUBMITTER: Vaidyanathan R 

PROVIDER: S-EPMC4470703 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature