Downregulation of CXCR4 in Metastasized Breast Cancer Cells and Implication in Their Dormancy.

ABSTRACT: Our understanding of the mechanism of cancer dormancy is emerging, but the underlying mechanisms are not fully understood. Here we analyzed mouse xenograft tumors derived from human breast cancer tissue and the human breast cancer cell line MDA-MB-231 to identify the molecules associated with cancer dormancy. In immunohistological examination using the proliferation marker Ki-67, the tumors included both proliferating and dormant cancer cells, but the number of dormant cells was remarkably increased when they metastasized to the lung. In the gene expression analysis of the orthotopic cancer cells by a single-cell multiplex real-time quantitative reverse transcription PCR followed by flow cytometric analysis, restrained cellular proliferation was associated with downregulation of the chemok

SUBMITTER: Nobutani K 

PROVIDER: S-EPMC4470829 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature