Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation.

ABSTRACT: Light-activatable proteins allow precise spatial and temporal control of biological processes in living cells and animals. Several approaches have been developed for controlling protein localization with light, including the conditional inhibition of a nuclear localization signal (NLS) with the Light Oxygen Voltage (AsLOV2) domain of phototropin 1 from Avena sativa. In the dark, the switch adopts a closed conformation that sterically blocks the NLS motif. Upon activation with blue light the C-terminus of the protein unfolds, freeing the NLS to direct the protein to the nucleus. A previous study showed that this approach can be used to control the localization and activity of proteins in mammalian tissue culture cells. Here, we extend this result by characterizing the binding properties of

SUBMITTER: Yumerefendi H 

PROVIDER: S-EPMC4471001 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature