Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association.

ABSTRACT: Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected microspore embryogenesis. By the use of Caramba ovaries it was possible to reach a 2-fold increase in the number of embryos and green plants, and to decrease the rate of albinism. Mature ovaries from flowers containing microspores at a late binucleate stage raised the number of embryos and green plants by 25-46% as compared to immature ovaries (excised from flowers with microspores at a mid-late unin

SUBMITTER: Castillo AM 

PROVIDER: S-EPMC4471355 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature