Suppression of problematic compound oligomerization by cosolubilization of nondetergent sulfobetaines.

ABSTRACT: Numerous small organic compounds exist in equilibrium among monomers, soluble oligomers, and insoluble aggregates in aqueous solution. Compound aggregation is a major reason for false positives in drug screening, and even soluble oligomers can interfere with structural and biochemical analyses. However, an efficient way to manage the equilibrium of aggregation-prone compounds, especially those involved with soluble oligomers, has not been established. In this study, solution NMR spectroscopy was used as a suitable technique to detect compound oligomers in equilibrium, and it was demonstrated that cosolubilization of nondetergent sulfobetaines (NDSBs) can largely suppress compound oligomerization and aggregation by shifting the equilibrium toward the monomers. The rotational correlation tim

SUBMITTER: Mizukoshi Y 

PROVIDER: S-EPMC4471626 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature