Time-lapse imaging assay using the BioStation CT: a sensitive drug-screening method for three-dimensional cell culture.

ABSTRACT: Three-dimensional (3D) cell culture is beneficial for physiological studies of tumor cells, due to its potential to deliver a high quantity of cell culture information that is representative of the cancer microenvironment and predictive of drug responses in vivo. Currently, gel-associated or matrix-associated 3D cell culture is comprised of intricate procedures that often result in experimental complexity. Therefore, we developed an innovative anti-cancer drug sensitivity screening technique for 3D cell culture on NanoCulture Plates (NCP) by employing the imaging device BioStation CT. Here, we showed that the human breast cancer cell lines BT474 and T47D form multicellular spheroids on NCP plates and compared their sensitivity to the anti-cancer drugs trastuzumab and paclitaxel using the B

SUBMITTER: Sakamoto R 

PROVIDER: S-EPMC4471796 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature