Pydna: a simulation and documentation tool for DNA assembly strategies using python.

ABSTRACT:

Background

Recent advances in synthetic biology have provided tools to efficiently construct complex DNA molecules which are an important part of many molecular biology and biotechnology projects. The planning of such constructs has traditionally been done manually using a DNA sequence editor which becomes error-prone as scale and complexity of the construction increase. A human-readable formal description of cloning and assembly strategies, which also allows for automatic computer simulation and verification, would therefore be a valuable tool.

Results

We have developed pydna, an extensible, free and open source Python library for simulating basic molecular biology DNA unit operations such as restriction digestion, ligation, PCR, primer design, Gibson assembly and homologou

SUBMITTER: Pereira F 

PROVIDER: S-EPMC4472420 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature