Airway epithelial NF-κB activation promotes the ability to overcome inhalational antigen tolerance.

ABSTRACT:

Background

Inhalational antigen tolerance typically protects against the development of allergic airway disease but may be overcome to induce allergic sensitization preceding the development of asthma.

Objectives

We examined in vivo whether pre-existing inhalational antigen tolerance could be overcome by activation of the transcription factor NF-κB in conducting airway epithelial cells, and used a combination of in vivo and in vitro approaches to examine the mechanisms involved.

Methods

Wild-type and transgenic mice capable of expressing constitutively active IκB kinase β (CAIKKβ) in airway epithelium were tolerized to inhaled ovalbumin. Twenty-eight days later, the transgene was transiently expressed and mice were exposed to inhaled OVA on Day 30 in an attempt to ove

SUBMITTER: Ather JL 

PROVIDER: S-EPMC4472492 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature